Atmospheric CO2 enrichment changes the wheat grain proteome

Publikations-Art

Zeitschriftenbeitrag (peer-reviewed)

Autoren

Högy, P., Zörb, C., Langenkämper, G., Betsche, T., Fangmeier, A.

Erscheinungsjahr

2009

Veröffentlicht in

Journal of Cereal Science

Band/Volume

50/

ISBN / ISSN / eISSN

DOI: 10.1016/j.jcs.2009.06.002

Seite (von - bis)

248-254

Spring wheat (Triticum aestivum L. cv. Triso) was grown in a free-air CO₂ enrichment (FACE) field experiment in order to gain information of CO₂-induced effects on grain composition and quality at maturity. A proteome analysis was performed using two dimensional gel electrophoresis (2-DE) and protein identification was done with mass spectrometry (MALDI-TOF MS). In elevated CO₂ (526 μl l^-1), an increase by 13.5% in grain yield was observed relative to 375 μl l^-1 at a low level of significance (P = 0.528). Total grain protein concentration was decreased by 3.5% at a high level of statistical significance. Most importantly, a number of statistically significant changes within the grain proteome were observed, as the levels of 32 proteins were affected by elevated CO₂: 16 proteins were up-regulated and 16 were down-regulated. Our experiment demonstrates that high-CO₂ can markedly affect the proteome of mature wheat grain. The potential role of the proteins, changed in response to CO₂ enrichment, is discussed as some may affect grain quality. For the task of selecting cultivars resistant against CO₂-induced quality loss, we propose to consider the proteins affected by elevated CO₂ identified in this work here.